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soilfungus/README.md

Gabriela Calcáneo-Hernández

Postdoctoral researcher at UNAM working on microbial evolution, comparative genomics, and hydrocarbon biodegradation.

CCC5EF3B-4D4C-434B-90A8-30FCD1DB8F3A

Pyrene-adapted Rhodococcus ruber strain

bitmap

Adaptive laboratory evolution of a petroleum-degrading bacterium under extreme pyrene selection followed by hybrid genome assembly, comparative genomics, and mutation analysis.

Available workflows

Genome Assembly

  • Unicycler hybrid assembly
  • Medaka polishing
  • Polypolish polishing
  • Assembly QC

Variant Analysis

  • breseq workflow
  • SNP calling using BWA, Samtools and BCFtools

RNA Structure Analysis

  • RNAfold analysis of non-coding mutations

Comparative Genomics

  • Plasmid comparison workflows
  • MUMmer alignments

Technical skills

  • Python
  • R
  • Linux
  • Git/GitHub
  • Genome assembly (Unicycler, Medaka, Polypolish)
  • Comparative genomics
  • Variant calling (breseq, BCFtools)
  • RNA secondary structure analysis (RNAfold)

Research interests

  • Comparative genomics
  • Adaptive laboratory evolution
  • Bioinformatics
  • Hydrocarbon biodegradation
  • Microbial physiology

Current projects

  • Comparative genomics of evolved strains
  • Genome assembly and polishing workflows

Publications

  • Journal of Fungi (2023) – DOI: 10.3390/jof9090939
  • Brazilian Journal of Microbiology (2020) – DOI: 10.1007/s42770-020-00329-7

📡 Find me

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  1. Pyrene-adapted-Rhodococcus-strain Pyrene-adapted-Rhodococcus-strain Public

    Bioinformatics workflows, Python scripts, and R templates developed during the adaptive laboratory evolution of Rhodococcus ruber under high pyrene concentrations. Includes genome assembly, polishi…

    Python