This software is developed to determine the fittest enzymes in Restriction Fragment Length Polymorphism (RFLP) analysis. The input data is a FASTA file from homogeneous organisms DNA sequences. The algorithm is able to rank enzymes that can distinguish RFLP patterns of the input sequences.
Place all the input sequences with FASTA format in a text file and name it as input_sequences.txt. Run the fitenz.m file in Matlab.