diff --git a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
index 1ac72cc86ee0..7519e7d58989 100644
--- a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
+++ b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
@@ -993,7 +993,7 @@ const definitions: FeatureFlagDefinitions = {
       ossReleaseStage: 'none',
     },
     animatedShouldUseSingleOp: {
-      defaultValue: false,
+      defaultValue: true,
       metadata: {
         dateAdded: '2024-02-05',
         description:
diff --git a/packages/react-native/src/private/animated/NativeAnimatedHelper.js b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
index 74a76280692a..eaaefb5b9a0b 100644
--- a/packages/react-native/src/private/animated/NativeAnimatedHelper.js
+++ b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
@@ -56,7 +56,8 @@ const singleOpQueue: Array<unknown> = [];
 const isSingleOpBatching =
   Platform.OS === 'android' &&
   NativeAnimatedModule?.queueAndExecuteBatchedOperations != null &&
-  ReactNativeFeatureFlags.animatedShouldUseSingleOp();
+  ReactNativeFeatureFlags.animatedShouldUseSingleOp() &&
+  !ReactNativeFeatureFlags.cxxNativeAnimatedEnabled();
 let flushQueueImmediate = null;
 
 const eventListenerGetValueCallbacks: {
diff --git a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
index ce445d0dc309..918b6b821aa1 100644
--- a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
+++ b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
@@ -4,7 +4,7 @@
  * This source code is licensed under the MIT license found in the
  * LICENSE file in the root directory of this source tree.
  *
- * @generated SignedSource<<c9804d0743d9a9d905193cb02816da46>>
+ * @generated SignedSource<<f44477b5f5a4f7030798a61694d0332a>>
  * @flow strict
  * @noformat
  */
@@ -164,7 +164,7 @@ export const animatedShouldSyncValueBeforeStartCallback: Getter<boolean> = creat
 /**
  * Enables an experimental mega-operation for Animated.js that replaces many calls to native with a single call into native, to reduce JSI/JNI traffic.
  */
-export const animatedShouldUseSingleOp: Getter<boolean> = createJavaScriptFlagGetter('animatedShouldUseSingleOp', false);
+export const animatedShouldUseSingleOp: Getter<boolean> = createJavaScriptFlagGetter('animatedShouldUseSingleOp', true);
 
 /**
  * Use the deferred cell render update mechanism for focus change in FlatList.